Pengaruh Pemberian Antibodi Monoklonal Human Zona Pelusida 3 (mAb HZP3) terhadap Jumlah Sel Granulosa pada Ovarium Mencit (Mus Musculus)
DOI:
https://doi.org/10.21776/ub.JOIM.2017.001.02.4Keywords:
imunokontrasepsi, antibodi monoklonal, ZP3Abstract
Antibodi ZP3 merupakan salah satu kandidat metode imunokontrasepsi. Penelitian sebelumnya mengevaluasi bahwa antibodi ZP3 dapat mengganggu fungsi gap junction sehingga mengganggu komunikasi dan transportasi growth factor antara sel granulosa dan oosit serta profil hormon. Sel granulosa memiliki peran yang sangat penting dalam folikulogenesis. Sel granulosa akan berproliferasi dan berdiferensiasi, menghasilkan hormon steroid dan mengekspresikan growth factor yang dibutuhkan untuk perkembangan oosit. Perkembangan sel granulosa yang terhambat dan tidak maksimal dapat menyebabkan perkembangan oosit yang tidak maksimal.Penelitian ini bertujuan untuk mengidentifikasi pengaruh pemberian Mab-hZP3 terhadap ekspresi KL di sel granulosa dan kadar FSH mencit (mus musculus). Metode penelitian adalah posttest only control group design. 48 ekor mencit dikelompokkan menjadi 12, yaitu kontrol (adjuvan), kelompok perlakuan (mAb hZP3 dosis 20 µg, 40 µg dan 60 µg), pengorbanan masing masing kelompok dilakukan pada hari ke 10, 15 dan 20. Penghitungan jumlah sel granulosa dilakukan dengan metode HE. Mencit yang diimunisasi dengan berbagai dosis Mab-hZP3 (dosis 20 µg, 40 µg dan 60 µg) dan diterminasi pada hari ke 10, 15 dan 20, jumlah sel granulosa tidak menunjukkan perbedaan tidak menunjukkan perbedaan yang signifikan. Mab-hZP3 diproduksi dengan menggunakan “bacterial system†dan berperan sebagai “spesific blockerâ€. Kesimpulannya Monoklonal antibodi human ZP3 tidak meningkatkan ekspresi KL dan kadar FSH. Hal ini memperkuat pengembangan Mab sebagai metode kontrasepsi alternatif yang aman.
References
BKKBN. 2014. Laporan Umpan Balik Pelayanan Kontrasepsi 2014. Kementerian Kesehatan Republik Indonesia: Jakarta.
Gupta, Satish. K., Gupta, N., Suman, P., Choudhury, S., Prakash, K., Gupta, T., Sriraman, R., Nagendrakumar, S.B., Srinivasan., V.A. 2011. Zona Pellucida-Based Contraceptive Vaccines for Human and Animal Utility. Journal of Reproductive Immunology. 88:240-246.
Spieler, J. 1987. Development of Immunological Methods of Fertility Regulation. Bulletin of The World Health Organization. 65 (6) : 779-783
Naz, R.K and Rajesh, C. 2004. Passive Immunization for Immunocontraception:. Lesson Learned from Infectious Diseases. Frontiers in Bioscience. 9(1): 2457-2465.
Barber, M.R. and Fayrer-Hosken, R.A. 2000. Possible Mechanism of Mammalian Immunocontraception. Journal of Reproductive Immunology. 46:103-124
East,.I.J., Mattison, D.R., and Dean, J. 1984. Monoclonal Antibodies to the Major Protein of the Murine Zona Pellucida: Effects on Fertilization and Early Development. Developmental Biology. 104: 49-56.
East,.I.J., Gulyas, B.J., and Dean, J. 1985. Monoclonal Antibodies to the Murine Zona Pellucida with Sperm Receptor Activity: Effects on Fertilization and Early Development. Developmental Biology. 109: 268-273.
Borillo, J., Coonrod, S.A., Wu J., Zhou, C. and Lou, Y. 2008. Antibodies to Two ZP3 B Cell Epitopes Affect Zona Pelusida Assembly. Journal of Reproductive Immunology. 78 (2):149-157.
Calongos, G., Hasegawa, A., Komori, S., and Koyama, K. 2009. Harmful Effects of Anti-Zona Pellucida Antibodies in Folliculogenesis, Oogenesis, and Fertilization. Journal of Reproductive Immunology. 79: 148-155.
Lloyd, M.L., Papadimitrou, J.M., O’Leary, S., Robertson, S.A., and Shellam, G.R. 2010. Immunoglobulin to Zona Pellucida 3 Mediates Ovarian Damage and Infertility after Contraceptive Vaccination in Mice. Journal of Autoimmunity. 35: 77-85.
Gershon, E., Placks, V., and Dekel, N. 2008. Gap Junction in The Ovary: Expression, Localization and Function. Molecular and Cellular Endocrinology. 282: 18-25.
Gittens, J.E.I., Barr, K.J., Vanderhyden, B.C., Kidder, G,M. 2004. Interplay Between Paracrine Signaling and Gap Junctional Communication in Ovarian Follicles. Journal of Cell Science. (1):114-122.
Kidder G.M., and Mhawi, A.A. 2002. Gap Junctions and Ovarian Folliculogenesis. Rerpoduction. 123: 613-620.
Celestino, J.J.H., Matos, M.H.T., Saraiva, M.V.A., and Figueiredo, J.R. 2009. Regulation of Ovarian Folliculogenesis by Kit Ligand and The c-Kit System in Mammals. Animal Reproduction. 6: 431-439.
Jones, R.L. and Pepling, M.E. 2013. KIT Signaling Regulates Primordial Follicle Formation in The Neonatal Mouse Ovary. Developmental Biology. 382: 186-197.
Sanchez, F., and Smitz, J. 2012. Molecular Control of Oogenesis. Biochimica et Biophysica Acta. 1822:1896-1912.
Strauss, F.J., Williams, J.C. 2014. The Ovarian Life Cycle in Yen and Jaffe’s Reproductive Endocrinology, 5th Ed; 213-253.
Tsunoda, y. And Chang, M.C. 1978. Effect of Antisera against Eggs and Zonae Pellucidae on Fertilization and Development of Mouse Eggs in vivo and Culture. J. Reprod. Fert. 54: 233-237
Hutt, K.J., McLaughlin, E.A., and Holland, M.K. 2006. Kit Ligand and c-Kit Have Diverse Roles During Mammalian Oogenesis and Folliculogenesis. Molecular Human Reproduction. 12: 61-69.
Dabbs, David. 2014. Diagnostic Immunohistochemistry: Theranostic and Genomic Applications. 4th Ed.
McPherson, R.A., and Pincus, M.R. 2011. Henry’s Clinical Diagnosis and Management Laboratory Methods. 22nd Ed.
Dahlan, Sopiyudin. 2013. Statistik untuk Kedokteran dan Kesehatan. Deskriptif, Bivariat dan Multivariat, Dilengkapi Aplikasi dengan menggunakan SPSS. Salemba Medika. Jakarta.
O’Leary, S., Lloyd, M.L., Shellam, G.R., and Robertson, S.A. 2008. Immunization with Recombinant Murine Cytomegalovirus Expressing Murine Zona Pellucida 3 Cause Permanent Infertility in BALB?c Mice Due to Follicle Depletion anf Ovulation Failure. Biology of Reproduction 79: 849-860.
Waldmann, T.A., and Stober, W.A. 1969. Metabolism of Immunoglobulins. Progr. Allergy. 13: 1-110.
